Fear Conditioning is a widely used paradigm to study associative emotional memory. An unconditional fear stimulus (foot shock) induces fear – displayed in form of freezing: the animal remains in a motionless, defensive posture. After one or several pairings of the foot shock with a neutral stimulus like a tone during training, this neutral stimulus becomes a conditional fear stimulus, which alone is then able to evoke a freezing response. This conditioned response - among others - is evaluated in the subsequent retention trial. The animal can be tested for its fear of the context and the conditioned stimulus/cue. Broadly applicable for animal models of Alzheimers, anxiety disorders and diseases with cognitive decline.