Water Maze

The Morris Water Maze paradigm

  • water maze
  • Morris Water Maze from TSE Systems

The Morris Water Maze paradigm, in which the animal has to learn the position of a submerged platform in a water pool by using extra-maze cues is widely used for testing spatial learning & memory. The hippocampus-dependent task detects cognitive deficits due to mutation, age or drug treatment.

The TSE Water Maze package includes a pool (diameter 120cm, 160cm or 180cm), a platform, a light-sensitive CCD camera, a mounting device, remote control and the video tracking software TSE VideoMot2. A customized tubing and pump set is available as an option. The pool is manufactured in black or white to achieve maximum contrast.

Quadrants and platform positions are drawn over the image of the pool. Use the remote control to start tracking immediately after the animal has been placed in the pool. The trial is stopped automatically when the animal has reached the platform (training only) or after a set duration has elapsed. Evaluate latency to reach the target in training trials. For probe trials the platform is removed – percent time spent and visits in the target quadrant are a measure of memory. Add parameters such as heading angle, Wishaw´s index or Thigmotaxis behavior to further differentiate between search strategies of animals.

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Baranowska U, Holownia A, Chabowski A, Baranowski M. Pharmacological inhibition of sphingosine-1-phosphate lyase partially reverses spatial memory impairment in streptozotocin-diabetic rats. Mol Cell Neurosci 2020; 107: 103526.

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Features

Complete package based on TSE VideoMot2.
Camera with USB connection to PC – no framegrabber required.
Detailed pre-configured analysis protocols.
Basic search strategy evaluation.
Pattern of movement, speed graph, heat map.
2 analysis licenses & 2 years free software update included.

Application

Spatial Learning & Memory.

Disease models

Alzheimer´s.
Cognition Deficits..

Compatible with