Hole Board

Exploration Test

  • Hole Board from TSE Systems

The Hole Board exploration test is a behavioral paradigm especially used in mice. The animal is placed on a floor equipped with a symmetrical arrangement of holes. Head dipping into these holes is used as a measure of exploration. The effect of stress, anxiety, or drugs targeting different neurotransmitter systems on head dipping behavior is evaluated.

The multi-purpose ActiMot2 system can be configured for Hole board experiments. A Hole Board with species-specific hole size is placed into an Open Field. With a grid of infrared sensors mounted below the board, hole visits are reliably detected using the Hole-Board plug-in of the comprehensive “ActiMot2 Advanced” software package. Gross activity on the board can be assessed by adding an additional sensor level that is located above the board. For detailed movement analysis on the board add a second 2-dimensional sensor frame and/or an optional rearing detector.

TSE Systems also provides a cost-effective stand-alone “Hole-Board-Only” software that can also be configured for a spatial version of the Hole-Board test that uses baited holes.

The Hole Board exploration test can also be configured in a Multi Conditioning setup.

Bouter Y, Brzózka MM, Rygula R, Pahlisch F, Leweke FM, Havemann-Reinecke U, Rohleder C. Chronic Psychosocial Stress Causes Increased Anxiety-Like Behavior and Alters Endocannabinoid Levels in the Brain of C57Bl/6J Mice. Cannabis Cannabinoid Res 2020; 5(1):51-61

Brzózka MM, Rossner MJ, de Hoz L. Tcf4 transgenic female mice display delayed adaptation in an auditory latent inhibition paradigm. Eur Arch Psychiatry Clin Neurosci 2016; 266(6): 505-12.


Quick and easy test on exploration.
No training required.
Reliable hole visit detection with height-adjustable infrared sensors.
Optional movement analysis on the board incl. rearing analysis.
Hole visits can be coupled to TTL output .

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